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A General Purification Procedure for Chemically Synthesized Oligoribonucleotides

✍ Scribed by J.B. Murray; A.K. Collier; J.R.P. Arnold

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
559 KB
Volume
218
Category
Article
ISSN
0003-2697

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✦ Synopsis

A general procedure for the purification of chemically synthesized oligoribonucleotides is reported. Purification based on the use of a single reverse-phase HPLC column with buffer systems of differing ion-pairing capacity is described. These methods have been applied to the preparation of a series of RNAs which range in size from 10 to 46 nucleotides. The yields obtained are high, up to (53 %) (based on isolated product compared to those obtained from final trityl assay). The purity of the isolated material is (96-99 %). Thus with this general procedure, milligram quantities of extremely pure RNA can be efficiently obtained. c 1994 Academic Press, tnc.

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