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A general one-step method for the cloning of PCR products

โœ Scribed by Angelo Bolchi; Simone Ottonello; Stefania Petrucco


Book ID
111718075
Publisher
Portland Press
Year
2005
Tongue
English
Weight
182 KB
Volume
42
Category
Article
ISSN
0885-4513

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The direct sequencing of the products of polymerase chain reactions (PCR) still presents difficulties and often requires special manipulations, such as the generation of excess single-stranded DNA using asymmetric PCR. Several alternative methods involve cloning PCR products into vector DNA suitable