A General Method for the Labeling of the Active Site of Antibodies and Enzymes
โ Scribed by Marian Elliott Koshland, Frieda Englberger and D. E. Koshland
- Book ID
- 123671349
- Publisher
- National Academy of Sciences
- Year
- 1959
- Tongue
- English
- Weight
- 289 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/89987
No coin nor oath required. For personal study only.
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The theoretical foundation has been laid for the investigation of catalytic systems using first-order kinetics and for a general kinetic method of investigation of the active site content, E(a), of enzymes, catalytic antibodies, and other enzyme-like catalysts. The method involves a combination of s
this way. TKe visualization of antibodies after electrofocusing exploits the high sensitivity possible with radioactive antigens without the problems of non-specific binding which sets a limit to the lowest level of antibody detectable by conventional antigen binding tests [ 111. The potential of th