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A general method for the detection of large CAG repeat expansions by fluorescent PCR.

✍ Scribed by Warner, J P; Barron, L H; Goudie, D; Kelly, K; Dow, D; Fitzpatrick, D R; Brock, D J


Book ID
118015435
Publisher
BMJ Publishing Group
Year
1996
Tongue
English
Weight
780 KB
Volume
33
Category
Article
ISSN
0022-2593

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Huntington disease (HD) is an autosomal dominant neurodegenerative disorder associated with expansions of an unstable CAG trinucleotide repeat in exon 1 of the IT15 gene. In normal individuals, IT15 contains up to 35 CAG repeats, while in affected the repeat length is >36. Polymerase chain reaction