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A fully automated and computerized system for simultaneous measurements of motility and phototaxis inChlamydomonas

✍ Scribed by Jürgen Pfan; Wilhelm Nultsch; Ursula Rüffer


Publisher
Springer
Year
1983
Tongue
English
Weight
914 KB
Volume
135
Category
Article
ISSN
0302-8933

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✦ Synopsis


A fully automated and computerized method for simultaneous measurements of motility and phototaxis of unicellular flagellates is described. Both systems are directly coupled with a homocontinuous culture. The motility measuring apparatus is equipped with a video camera and recorder for simultaneous single cell behaviour studies. First results of studies on the effects of the phototaxis inhibitor sodium azide and the Ca 2 + conducting ionophore A 23 187 on motility and phototaxis of Chlamydomonas are reported and correlated with video observations. These results demonstrate that the described systems give informations of whether phototaxis or motility or both are inhibited by chemicals.


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