Simultaneous determination of gemfibrozil and its metabolites in plasma and urine by a fully automated high performance liquid chromatographic system

✍ Scribed by Akihiko Nakagawa; Akemi Shigeta; Haruo Iwabuchi; Masaaki Horiguchi; Kan-ichi Nakamura; Hidekuni Takahagi

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 542 KB
• Volume: 5
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130050205

✦ Synopsis

Sensitive and specific methods for the simultaneous determination of gemfibrozil (Lopid@), a lipid-lowering agent, and its metabolites in plasma and urine are described. The methods are based on a fully automated high performance liquid chromatographic (HPLC) system with fluorescence detection. Urine samples, diluted with acetonitrile, were directly analysed by HPLC using a flow and eluent programming method. In the case of plasma, gemfibruzil and its main metabolites were extracted from acidified samples and the resulting extracts injected into the chromatographic system. The sensitivity was approximately 100 ng/mL for gemfibrozil and its four metabolites using 0.5 mL plasma or urine. An acyl glucuronide of gemfibrozil excreted in human urine after oral administration of the drug was isolated and its structure and stability examined.