A method is described for rapidly quenching metabolism in yeast and extracting metabolites for analysis. The saponin digitonin is used to permeabilize the yeast cell membrane, in conjunction with perchloric acid (PCA) to quench metabolism and extract metabolites. Using this digitonin-PCA quench and
A fluorometric method for the enzymic determination of glycolytic intermediates
โ Scribed by P.K. Maitra; R.W. Estabrook
- Publisher
- Elsevier Science
- Year
- 1964
- Tongue
- English
- Weight
- 775 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0003-2697
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