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A fluorometric method for the determination of 2-deoxy-d-glucose

โœ Scribed by Melvin Blecher


Book ID
107710468
Publisher
Elsevier Science
Year
1961
Tongue
English
Weight
458 KB
Volume
2
Category
Article
ISSN
0003-2697

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A Fluorometric Assay for the Determinati
โœ Jordi Querol; Oscar Besumbes; Luisa Maria Lois; Albert Boronat; Santiago Imperia ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 64 KB

We report a novel fluorometric end-point assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity based on the reaction of 1-deoxy-D-xylulose 5-phosphate (DX5P) with 3,5-diaminobenzoic acid in an acidic medium to form a highly fluorescent quinaldine derivative. The assay