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A fluorometric method for determining the degree of biotinylation of proteins

โœ Scribed by G.P. Der-Balian; B. Gomez; R.S. Masino; J.W. Parce


Book ID
118062218
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
324 KB
Volume
126
Category
Article
ISSN
0022-1759

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Digestion of proteins with a mixture of chymotrypsin and pronase followed by dilution in 6 M urea eliminates the quenching effects usually observed when tryptophan fluorescence is measured in native or denatured proteins. Following proteolysis, the tryptophan content can be estimated from the fluore