A fluorometric method for the determinat
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T. Sasaki; B. Abrams; B.L. Horecker
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Article
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1975
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Elsevier Science
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English
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Digestion of proteins with a mixture of chymotrypsin and pronase followed by dilution in 6 M urea eliminates the quenching effects usually observed when tryptophan fluorescence is measured in native or denatured proteins. Following proteolysis, the tryptophan content can be estimated from the fluore