A fluorimetric assay for angiotensin-I converting enzyme in human serum
β Scribed by Amos Carmel; Sarah Ehrlich-Rogozinsky; Arieh Yaron
- Book ID
- 115822542
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 436 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
Furanacryloyl tripeptides conforming to the known substrate specificity of the angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1) have been employed to provide a continuous spectrophotometric assay for this peptidase in the visible region. The assay is based on a blue shift of
A simple and sensitive assay for angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity has been developed which employs fluorescently labeled tripeptides. ACE hydrolyzes dansylphenylalanyl-arginyl-tryptophan or dansyl-phenylalanyl-arginyl-phenylalanine, liberating dansyl-phenylalanine and a dipe