𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A continuous spectrophotometric assay for angiotensin converting enzyme

✍ Scribed by Barton Holmquist; Peter Bünning; James F. Riordan

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
701 KB
Volume
95
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Furanacryloyl tripeptides conforming to the known substrate specificity of the angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1) have been employed to provide a continuous spectrophotometric assay for this peptidase in the visible region. The assay is based on a blue shift of the absorption spectrum that occurs upon hydrolysis of the substrate to produce a furanacryloyl-blocked amino acid and a dipeptide. Of the various furanacryloyl tripeptides tested, furanacryloyl+phenylalanylglycylglycine exhibits the most suitable characteristics for routine assays of angiotensin converting enzyme.

📜 SIMILAR VOLUMES

A rapid and simple spectrophotometric as
A rapid and simple spectrophotometric assay of angiotensin-converting enzyme
✍ Makoto Hayakari; Yoshikazu Kondo; Hiroshi Izumi 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 425 KB

A rapid, simple, and accurate method for the chemical assay of angiotensin-converting enzyme has been developed. The method relies on previously published method for spectrophotometric assay of angiotensinconverting enzyme activity and on the use of 2,4,6-trichloro-s-triazine (TT) as a calorimetric

A fluorometric assay for angiotensin-con
A fluorometric assay for angiotensin-converting enzyme activity
✍ Michael S. Kapiloff; Stephen M. Strittmatter; Lloyd D. Fricker; Solomon H. Snyde 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 850 KB

A simple and sensitive assay for angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity has been developed which employs fluorescently labeled tripeptides. ACE hydrolyzes dansylphenylalanyl-arginyl-tryptophan or dansyl-phenylalanyl-arginyl-phenylalanine, liberating dansyl-phenylalanine and a dipe

Assays for Angiotensin Converting Enzyme
Assays for Angiotensin Converting Enzyme Inhibitory Activity
✍ Bin-Wha Chang; Richie L.C. Chen; I-Jen Huang; Hsien-Chang Chang 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 66 KB

A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidyl-leucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM(-1) cm(-1)) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogeni

An Assay for Angiotensin-Converting Enzy
An Assay for Angiotensin-Converting Enzyme Using Capillary Zone Electrophoresis
✍ Rong-zhen Zhang; Xiao-hua Xu; Tian-bao Chen; Long Li; Ping-fan Rao 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 62 KB

A sensitive and rapid method was developed for angiotensin-converting enzyme (ACE) activity determination by capillary zone electrophoresis. Hippuryl-l-histidyl-l-leucine, a synthetic tripeptide, was used as the ACE-specific substrate. Capillary zone electrophoresis was employed to separate the prod