𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A fluorescent method for the demonstration of cyclic 3′, 5′-AMP phosphodiesterase activity in polyacrylamide gel

✍ Scribed by K.C. Tsou; K.W. Lo; K.F. Yip

Book ID
115904739
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
253 KB
Volume
45
Category
Article
ISSN
0014-5793

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A fluorogenic method for the demonstrati
A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis
✍ K.W. Lo; S. Aoyagi; K.C. Tsou 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 687 KB

A rapid fluorogenic method for the demonstration of S-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl Sthymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C. and the sens

Mutants ofSerratia marcescenslacking cyc
Mutants ofSerratia marcescenslacking cyclic nucleotide phosphodiesterase activity and requiring cyclic 3′,5′-AMP for the utilization of various carbohydrates
✍ U. Winkler; H. Scholle; L. Bohne 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 866 KB

Adenine requiring mutants of Serratia marcescens SM-6-F'lac + have been found to grow well in minimalglucose medium solely supplemented with cAMP. From one of these ade strains double mutants (called ade cpd) were isolated which could no longer utilize cAMP but which still grew on 5'AMP. Dialyzed ce