𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Fluorescence Quenching Method for the Determination of Nitrite with Indole

✍ Scribed by Nianqin Jie; Duanling Yang; Quibin Jiang; Qiang Zhang; Lu Wei

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
41 KB
Volume
62
Category
Article
ISSN
0026-265X

No coin nor oath required. For personal study only.

✦ Synopsis

A new fluorescence quenching method has been developed to determine nitrite in water and food samples. The method is based on the reaction between nitrite and the fluorescent indole to form a compound which has no fluorescence in acidic medium. The fluorescence intensity was measured in 1 cm quartz cell with excitation and emission wavelengths of 285 and 350 nm, respectively. The relationship was obtained between the fluorescence intensity and nitrite concentration in the range 0.01-0.6 g ml Οͺ1 . The detection limit was 2.5 ng ml Οͺ1 . The proposed method was applied to determine nitrite in water and food samples. The mechanism involved in the reaction was studied.

πŸ“œ SIMILAR VOLUMES

Fluorescence quenching method for the de
Fluorescence quenching method for the determination of bleomycins A5 and A2 with halofluorescein dyes
✍ Jiangtao Liu; Zhongfang Liu; Xiaoli Hu; Ling Kong; Shaopu Liu πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 265 KB πŸ‘ 2 views

In weak acidic medium, the anticancer antibiotics bleomycin A5 (BLMA5) and bleomycin A2 (BLMA2) bind with halofluorescein dyes, such as erythrosin (Ery), eosin Y (EY) and eosin B (EB), to form ion-association complexes, which causes fluorescence quenching of halofluorescein dyes. The quenching value