A Fluorescence Energy Transfer Method for Analyzing Protein Oligomeric Structure: Application to Phospholamban
β Scribed by Li, Ming; Reddy, Laxma G.; Bennett, Roberta; Silva, Norberto D.; Jones, Larry R.; Thomas, David D.
- Book ID
- 119412384
- Publisher
- Biophysical Society
- Year
- 1999
- Tongue
- English
- Weight
- 225 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0006-3495
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Section I. Determination, Detection, And Localization -- Section Ii. Interacting Proteins And Subunits -- Section Iii. Inhibition, Stimulation, And Modulation Of Activity -- Section Iv. Expression Systems -- Section V. Knockdown And Knockout Technologies. Edited By Susanne Klumpp, Josef Krieglstein.
A sensitive fluorescence resonance energy transfer method was developed for the direct measurement of the dissociation constants of stromelysin inhibitors. The method is applied to the thiadiazole class of stromelysin inhibitors and it takes advantage of the fact that, upon binding to the active sit