[Methods in Enzymology] Protein Phosphatases Volume 366 || The Application of Fluorescence Resonance Energy Transfer to the Investigation of Phosphatases
✍ Scribed by SzöllÖsi, János
- Book ID
- 120936126
- Publisher
- Elsevier
- Year
- 2003
- Tongue
- English
- Weight
- 287 KB
- Edition
- 1
- Category
- Article
- ISBN
- 0121822699
- ISSN
- 0076-6879
No coin nor oath required. For personal study only.
✦ Synopsis
Section I. Determination, Detection, And Localization -- Section Ii. Interacting Proteins And Subunits -- Section Iii. Inhibition, Stimulation, And Modulation Of Activity -- Section Iv. Expression Systems -- Section V. Knockdown And Knockout Technologies. Edited By Susanne Klumpp, Josef Krieglstein. Includes Bibliographical References And Indexes.
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Enhanced blue fluorescent protein (EBFP) and enhanced green fluorescent protein (EGFP) mutants of GFP in close proximity to one another can act as a fluorescence resonance energy transfer (FRET) pair. Unstructured amino acid linkers of varying length were inserted between EBFP and EGFP, revealing th