A fluorescence-based assay for N-myristoyltransferase activity
โ Scribed by Victor Goncalves; James A. Brannigan; Emmanuelle Thinon; Tayo O. Olaleye; Remigiusz Serwa; Salvatore Lanzarone; Anthony J. Wilkinson; Edward W. Tate; Robin J. Leatherbarrow
- Book ID
- 113412276
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 309 KB
- Volume
- 421
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We developed an assay to test for inhibition of myristoyl-CoA:protein \(\mathbf{N}\)-myristoyltransferase (NMT) activity since this enzyme is important in viral replication and cellular biochemistry. Saccharomyces cerevisae NMT was harvested from Escherichia coli carrying a plasmid vector containing
Site-specific attachment of hydrophobic moieties to proteins (fatty acid acylation and prenylation) is a functionally essential modification involved in intracellular signaling pathways (1-3). Study of the transferase enzymes that catalyze these modifications is complicated by troublesome single poi