In the EM-routine, the thin sections are usually attached onto round copper grids. The sections on these EM-grids are then stained either with a quite expensive automatic staining apparatus or by transporting the grids by hand from solution to solution. Here we will present a simple, inexpensive and
A flow-through staining system for use with multiple em grids
β Scribed by Stetson, D. L. ;Morrone, M.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1989
- Tongue
- English
- Weight
- 132 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0741-0581
No coin nor oath required. For personal study only.
β¦ Synopsis
The SynapTek@ Gridstick Kit offers a convenient system for staining multiple grids for transmission electron microscopy. However, the system is not convenient for filtering staining solutions and for minimizing atmospheric exposure during the staining process. Because we have experienced considerable difficulties in eliminating stain contamination, we modified the SynapTek system to provide for double filtering of solutions, while eliminating exposure to air as solutions are changed.
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