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A flow microcalorimetric study of the inhibition of acetylcholinesterase by catecholamine derivatives

✍ Scribed by Cristina M.P. Silva; M.Conceiçäo P. De Lima; Catarina R. Oliveira; Arséio P. Carvlho


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
613 KB
Volume
179
Category
Article
ISSN
0040-6031

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A flow injection system for determination of metrifonate by inhibition of immobilised acetylcholinesterase (AChE) is described. Acetylthiocholine is hydrolysed by AChE, and the thiocholine produced is mixed with the chromogen 5,5dithiobis(2-nitrobenzoic acid) and the absorbance is measured at 405nm.