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A flow cytometric protocol for enumeration of endothelial progenitor cells and monocyte subsets in human blood

✍ Scribed by Mihail Hristov; Susanne Schmitz; Frans Nauwelaers; Christian Weber

Book ID: 111692199
Publisher: Elsevier Science
Year: 2012
Tongue: English
Weight: 934 KB
Volume: 381
Category: Article
ISSN: 0022-1759
DOI: 10.1016/j.jim.2012.04.003

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Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w