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A few experiments with thin-layer chromatography on charcoal

✍ Scribed by Želimír Procházka

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
460 KB
Volume
48
Category
Article
ISSN
1873-3778

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✦ Synopsis

Plates for thin-layer chromatography on charcoal were prepared using polyethylene as binder. The problem of detection was overcome in several ways and the adsorptive powers of silica gel and charcoal layers were compared using two sets of substances: dyes and amino acids. The charcoal employed was a stronger adsorbent than silica gel. The RF data of the substances mentioned, in severalsolvents, are represented as chromatographic profiles.

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A thin layer chromatography experiment of medical importance is described. The experiment involves extraction of lipids from simulated amniotic fluid samples followed by separation, detection, and scanning of the lecithin and sphingomyelin bands on TLC plates. The lecithin-to-sphingomyelin ratio is