✦ LIBER ✦

A direct enzymatic synthesis of β-d-galactopyranosyl-d-xylopyranosides and their use to evaluate rat intestinal lactase activity in vivo

✍ Scribed by Juan J. Aragón; Francisco J. Cañada; Alfonso Fernández-Mayoralas; Rosa López; Manuel Martín-Lomas; Daniel Villanueva

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 422 KB
Volume: 290
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(96)00113-9

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✦ Synopsis

By enzymatic fl-o-galactosylation of D-xylose a mixture of 4-, 3-, and 2-O-fl-D-galactopyranosyl-D-xyloses (1, 4, and 7, respectively) was obtained in 50% isolated yield. Disaccharides 1, 4, and 7 are substrates of intestinal lactase isolated from lamb small intestine with K m values of 250.0, 4.5, and 14.0 mM, respectively. The mixture was used to monitor the normal decline in lactase activity in rats that takes place after weaning. The data obtained by this method correlated with the levels of intestinal lactase activity in the same animals after being sacrificed.

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