Mass production of thermostable D-hydant
โ
Dong-Cheol Lee; Geun-Joong Kim; Yoo-Kyong Cha; Cheo-Young Lee; Hak-Sung Kim
๐
Article
๐
1997
๐
John Wiley and Sons
๐
English
โ 134 KB
D-Hydantoinase is an industrial enzyme widely used for the synthesis of optically active D-amino acids. A gene encoding thermostable D-hydantoinase of Bacillus stearothermophilus SD-1 has previously been cloned and constitutively expressed by its native promoter in Escherichia coli XL1-Blue (Lee et