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A counter-selectable marker for genetic transformation of the yeastSchwanniomyces alluvius

✍ Scribed by M. N. Dave; B. B. Chattoo

Publisher
Springer
Year
1997
Tongue
English
Weight
262 KB
Volume
48
Category
Article
ISSN
1432-0614

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A plasmid (pAmN61) containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts. Neomycin resistant transformants could be selected for on complete medium containing 1.2 mg/