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A convenient method for the determination of the optical configuration of cystathionine

✍ Scribed by Tadashi Ogawa; Masumi Kimoto; Kei Sasaoka

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
287 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

A convenient and sensitive method was developed for the determination of the optical configuration of cystathionine in microscale. The technique consists of the reductive desulfuration of cystathionine to alanine and a-amino-n-butyric acid with Raney nickel and the chromatographic resolution of the diastereoisomeric L-leucyl dipeptides of both the desulfuration products. Examples used for cystathionine isolated from natural sources, a commercial product, and a synthetic specimen were presented.

MATERIALS AND METHODS

Materials.

L-Cystathionine (Lot No. 127C-3780) was purchased from Sigma

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## Abstract A novel and very accurate method was established for the determination of the optical purity of a peptide by use of the following procedure: (1) hydrolysis of the peptide in deuterium chloride, (2) gas chromatographic separation of each amino acid enantiomer on a chiral phase, and (3) d