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A convenient and sensitive method for the determination of serum aspartate aminotransferase isozymes after electrophoresis

✍ Scribed by Sakakibara Shigeki; Shiomi Katsuhiko; Kobayashi Shigeko; Ikeda Toshiko; Inai Shinya; Kagamiyama Hiroyuki

Book ID
115824176
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
379 KB
Volume
133
Category
Article
ISSN
0009-8981

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✍ K.W. Lo; S. Aoyagi; K.C. Tsou πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 687 KB

A rapid fluorogenic method for the demonstration of S-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl Sthymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25Β°C. and the sens