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A continuous-flow system for the growth of RNA tumor viruses

✍ Scribed by Kenneth F. Manly

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
554 KB
Volume
63
Category
Article
ISSN
0003-2697

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✦ Synopsis

By continuously pumping tissue culture medium into roller bottles containing infected cells and continuously harvesting the spent medium. RNA tumor virus can be harvested conveniently within 20 min of its release from the infected cells. An apparatus used to collect Moloney mouse leukemia virus in such a way yielded up to 390 pg protein in purified virus (about I mg virus) from each liter of tissue culture fluid.

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