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A comprehensive evaluation of normalization methods for Illumina high-throughput RNA sequencing data analysis

โœ Scribed by Dillies, M.-A.; Rau, A.; Aubert, J.; Hennequet-Antier, C.; Jeanmougin, M.; Servant, N.; Keime, C.; Marot, G.; Castel, D.; Estelle, J.; Guernec, G.; Jagla, B.; Jouneau, L.; Laloe, D.; Le Gall, C.; Schaeffer, B.; Le Crom, S.; Guedj, M.; Jaffrezic, F.


Book ID
121532147
Publisher
Oxford University Press
Year
2012
Tongue
English
Weight
386 KB
Volume
14
Category
Article
ISSN
1467-5463

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The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq is likely to become the platform of choice for interrogating steady state RNA. In order to discover biologically important changes in expression, we show that normalization continues to be an essential step in t