A comparison of the enzyme fluorometric and the peroxyoxalate chemiluminescence methods for measuring H2O2
β Scribed by N. Beltz; W. Jaeschke; G. L. Kok; S. N. Gitlin; A. L. Lazrus; S. McLaren; D. Shakespeare; V. A. Mohnen
- Book ID
- 104625705
- Publisher
- Springer Netherlands
- Year
- 1987
- Tongue
- English
- Weight
- 533 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0167-7764
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β¦ Synopsis
Both methods showed excellent agreement, with a maximum deviation of _+5%. Only at unusually high concentrations of some potential atmospheric species could slight interferences be observed. During the experiments the detection limits were 1.3 Γ 10 -8 mol/l (0.44 ppbm) of H202 for the fluorometric instrument and 4 Γ l0 -~ mol/1 (1.36 ppbm) of H202 for the chemiluminescence instrument. For the chemiluminescence technique, the response to methylhydroperoxide was approximately 80-fold less than that to an equivalent concentration of H 2 02.
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Emissions at 450 nm and 4.27 ~sm have been measured when a vaxiety of mixtures containing H 2, CO. either 02 or NaO, and Ar were heated behind reflected shock waves to temperatures of 2000-2850 K and total concentrations near 5 x 101| molecule/era 3. These emissions were used to obtain absolute con
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