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A comparison of the enzyme fluorometric and the peroxyoxalate chemiluminescence methods for measuring H2O2

✍ Scribed by N. Beltz; W. Jaeschke; G. L. Kok; S. N. Gitlin; A. L. Lazrus; S. McLaren; D. Shakespeare; V. A. Mohnen


Book ID
104625705
Publisher
Springer Netherlands
Year
1987
Tongue
English
Weight
533 KB
Volume
5
Category
Article
ISSN
0167-7764

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✦ Synopsis


Both methods showed excellent agreement, with a maximum deviation of _+5%. Only at unusually high concentrations of some potential atmospheric species could slight interferences be observed. During the experiments the detection limits were 1.3 Γ— 10 -8 mol/l (0.44 ppbm) of H202 for the fluorometric instrument and 4 Γ— l0 -~ mol/1 (1.36 ppbm) of H202 for the chemiluminescence instrument. For the chemiluminescence technique, the response to methylhydroperoxide was approximately 80-fold less than that to an equivalent concentration of H 2 02.


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