A comparison of the activities of the products of the two genes for elongation factor Tu
β Scribed by Miller, David Lee ;Nagarkatti, Surekha ;Laursen, Richard A. ;Parker, Jack ;Friesen, James D.
- Book ID
- 104758252
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 815 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
The two species of Elongation Factor Tu coded for by the tufA and tufB genes were synthesized in UV-irradiated E coli infected by transducing phages bearing the separate genes. Both proteins interact similarly with EFTs, GDP, and phe-tRNA. Although the phe-tRNA.EFTu.GMP.PNP complex containing the tufA gene product binds somewhat more tightly to ribosomes, both proteins promote the complete process of binding phe tRNA to ribosomes at similar rates.
π SIMILAR VOLUMES
Two crystal forms of a complex between trypsin-modified elongation factor Tu-MgGDP from Escherichia coli and the antibiotic tetracycline have been solved by X-ray diffraction analysis to resolutions of 2.8 and 2.1 A, respectively. In the P2(1) form, cocrystals were grown from a solution mixture of t