Methods are given for the quantitation of the plant stress hormone, abscisic acid (ABA), and its two metabolic precursors, ketone and enolate, that are applicable to all species tested so far. The plant extract is homogenized at neutral pH, hexane-soluble neutrals are extracted and discarded, and th
A comparison of some methods for the purification of abscisic acid and its metabolites from plants extracts
โ Scribed by George J. P. Murphy
- Publisher
- Springer Netherlands
- Year
- 1985
- Tongue
- English
- Weight
- 365 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0167-6903
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โฆ Synopsis
The use of small columns containing charcoal and cellulose for the purification of abscisic acid and its metabolites from ptant extracts is described. These columns have high capacity and the sample processing time is short. The efficiencies of recovery of abscisic acid, abscisic acid glucose ester, dihydrophaseic acid and an unidentified highly polar metabolite of abscisic acid are about 90%. The method is shown to be superior to other published methods using commercial pre-packed cartridges.
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