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A Comparison of Measured and Calculated Single- and Double-Stranded Oligodeoxynucleotide Extinction Coefficients

✍ Scribed by Gary Kallansrud; Brian Ward


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
94 KB
Volume
236
Category
Article
ISSN
0003-2697

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo