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A comparison of interfertility and in vitro DNA-DNA reassociation as criteria for speciation in the genusKluyveromyces

✍ Scribed by J. P. Walt; Elzbieta Johannsen

Book ID
104767467
Publisher
Springer Netherlands
Year
1979
Tongue
English
Weight
663 KB
Volume
45
Category
Article
ISSN
0003-6072

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✦ Synopsis

Hybridization studies based on the use of the prototrophic selection technique were undertaken to compare interfertility and DNA-DNA reassociation as criteria for speciation purposes in the yeast genus Kluyveromyces. Degrees of DNA-DNA reassociation greater than 70% between strains, as reported in the literature, were found to correlate with high recombination frequencies. Degrees of DNA-DNA reassociation less than 20% between strains did, however, not invariably coincide with the absence of interfertility between strains. If interfertility is accepted as criterion for conspecificity, the hitherto reported low degrees (less than 20%) of DNA-DNA reassociation in Kluyveromyces cannot confidently be employed for speciation purposes.

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## COMMUNICATIONS TO THE EDITOR The Magnitude of Intramolecular DNA Optical Absorbance Special Application to DNA Reassociation Studies with Variation in "a+] and GC Base Composition: For ## Synopsis The magnitude of intramolecular DNA optical absorbance is presented as a function of "a+] and G