The magnitude of intramolecular DNA optical absorbance with variation in [Na+] and GC base composition: For special application to DNA reassociation studies
✍ Scribed by Kenneth A. Marx
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1975
- Tongue
- English
- Weight
- 310 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
COMMUNICATIONS TO THE EDITOR
The Magnitude of Intramolecular DNA Optical Absorbance Special Application to DNA Reassociation Studies with Variation in "a+] and GC Base Composition: For
Synopsis
The magnitude of intramolecular DNA optical absorbance is presented as a function of "a+] and GC composition of the DNA. The data are presented as a ratio of absorbances A o T r / A o T d for the DNA at the denaturing temperature (Td) and renaturing temperature (T,) under the given conditions. All ratios were determined for T , corresponding to the temperature optimum ( T , -T , = 25OC) in DNA reassociation rate. This fact, coupled with the convenient AoTr/AoTd ratio representation, permits the quick estimation of the magnitude of this optical effect in DNA reassociation reactions over a wide range of experimental conditions.