A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels

✍ Scribed by Guang-Shing Perng; R.Dino Rulli; David L. Wilson; G.W. Perry

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 418 KB
• Volume: 173
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90204-7

✦ Synopsis

Eight different methods of fluorographic enhancement of sensitivity to "S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of "S after a l-week exposure.