Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in the Giemsa methods. The concentration of Hoechst 33258, pH of the mounting medium temperature during UV-exposure and the quality (wavelength)of UV-light influe
A combination of sister chromatid differential staining and giemsa banding
โ Scribed by S. Pathak; A. D. Stock; A. Lusby
- Book ID
- 112738358
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 721 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1420-682X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method is presented for obtaining a combination of differential sister chromatid staining. G-banding and X-chromosome inactivation pattern. The result of this method enables a precise localization of the sister chromatid exchange points to particular bands of individual chromosomes.
The direct staining of BrdU-substituted Chinese hamster chromosomes in a Na2HPO4-Giemsa solution without any pretreatments resulted in a B-dark type SCD istituted (TB) chromatids stained light. Detailed examinations of the staining process suggested that the Na2HPO4 solution acts to collapse chromos
The essential steps of the 33258 Hoechst-Giemsa method for differential chromatid staining consist of (1) 33258 Hoechst treatment, (2) exposure to light, and (3) Giemsa staining. The staining was shown to be a function of the concentration of 33258 Hoechst and the light exposure. The dye was success