New Giemsa method for the differential staining of sister chromatids
โ Scribed by PERRY, PAUL; WOLFF, SHELDON
- Book ID
- 109692723
- Publisher
- Nature Publishing Group
- Year
- 1974
- Tongue
- English
- Weight
- 562 KB
- Volume
- 251
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/251156a0
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Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in the Giemsa methods. The concentration of Hoechst 33258, pH of the mounting medium temperature during UV-exposure and the quality (wavelength)of UV-light influe
The DNA labelling of the bifilarly 5-bromodeoxyuridine- to-substituted chromatid decreased relative to that of the unifilarly substituted chromatid with increasing duration of HB pretreatment (Hoechst 33258 plus black light at 55 degrees C). Sister chromatid differential staining was detected by Gie