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A colorimetric determination of the number of eelworms in a suspension

โœ Scribed by L. H. J. Korsten; J. W. Sieben; L. Voskuyl

Publisher
Springer
Year
1953
Tongue
English
Weight
188 KB
Volume
2
Category
Article
ISSN
0014-2336

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โœฆ Synopsis

Artificial infestation of red clover seedlings with stem eelworms (Ditylenchus dipsaci KiiHN) has shown that the appearance of clear symptoms of susceptibility -thickened stems -is dependent on the number of eelworms that penetrate the plants . In order to obtain clear symptoms, 2-8 eelworms to each plant are sufficient . Large numbers of eelworms give rise to necrosis and death of the plants . In testing for resistance to stem eelworm in the breeding of clovers it is desirable to infest lightly and always to use inocula of the same concentration.

In this article attention is called to a quick and reliable method of determining the eelworm concentration in a suspension used for inoculation .

(It is assumed that the average size of the eelworms differs very little) .

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