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A colorimetric assay for the determination of 5′-nucleotidase activity

✍ Scribed by S. Perez; N. Courtis; D. Kokkinopoulos; M. Papamichail; C.M. Tsiapalis; T. Trangas

Book ID
118900708
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
393 KB
Volume
101
Category
Article
ISSN
0022-1759

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A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified