A class of ompA mutants of Escherichia coliK12 affected in the interaction of OmpA protein and the core region of lipopolysaccharide

✍ Scribed by Puspurs, Arnis ;Medon, Peter ;Corless, Chris ;Hackett, Jim ;Reeves, Peter

• Book ID: 104722846
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 409 KB
• Volume: 189
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00326070

✦ Synopsis

A group of ompA mutants of Escherichia coli K12 are described which were sensitive to bacteriophage K3 in a background wild-type for lipopolysaccharide (LPS). With mutant LPS in vivo (lacking some core sugar residues), however, the ompA mutations gave resistance to K3. Outer membrane levels of OmpA protein were normal or near-normal when the mutations resided in either wild-type or mutant LPS backgrounds. Strains in which the mutations occurred in a wild-type LPS background adsorbed K3 phage at the same initial rate and to the same extent as a wild-type strain, but the efficiency of plaquing of the adsorbed K3 was reduced to 25-50% of wild-type levels. Under conditions where a wild-type strain irreversibly adsorbed over 90% of available phage K3 within 3 min, double mutants (ompA mutant, LPS mutant) left 90% of the phage viable after 1 h. The 10% of inactivated phage did not form plaques.