Precursor Proteins Are Intermediates in vivo, in the Synthesis of Two Major Outer Membrane Proteins, the OmpA and OmpF Proteins, of Escherichia coli K12
โ Scribed by Ian CROWLESMITH; Konrad GAMON; Ulf HENNING
- Book ID
- 115119731
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 675 KB
- Volume
- 113
- Category
- Article
- ISSN
- 1432-1327
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The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea
To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat