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A circular dichroism study of poly dG, poly dC, and poly dG:dC

✍ Scribed by Donald M. Gray; F. J. Bollum

Publisher: Wiley (John Wiley & Sons)
Year: 1974
Tongue: English
Weight: 898 KB
Volume: 13
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1974.360131011

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✦ Synopsis

Abstract

We have measured the ultraviolet circular dichroism spectra of oligo d(pG)~5~, poly dN AcG, poly dI, poly dC, two samples of poly dG, and four samples containing double‐stranded poly dG:dC. We find that oligo d(pG)~5~ and poly dG exist in self‐complexed forms as well as in single‐stranded forms. Unlike the self‐complexed form of poly dG, the single‐stranded form of poly dG can hydrogen‐bond with single‐stranded poly dC. We present spectral data for double‐stranded poly dG:dC, which can be used to help characterize poly dG:dC preparations and which provide a basis for resolving discrepancies among other reported poly dG:dC spectra.

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