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A chromosome painting method for human sperm chromosomes using fluorescentin situhybridization

✍ Scribed by Xiao Cui; Hiroyuki Tateno; Isamu Hayata; Koki Sato; Yujiroh Kamiguchi


Book ID
105333895
Publisher
Nature Publishing Group
Year
1994
Tongue
English
Weight
553 KB
Volume
39
Category
Article
ISSN
1435-232X

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A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localiz