✦ LIBER ✦
An improved method for chromosome banding after fluorescencein situhybridization: Use of a tetramethylrodhamine-conjugated BrdU antibody
✍ Scribed by Albertina Sario; Luigi Carli; Elena Raimondi
- Book ID
- 104635935
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 557 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0920-9069
No coin nor oath required. For personal study only.
✦ Synopsis
A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.