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A Broadened Scope for the Use of Hydrazones as Neutral Nucleophiles in the Presence of H-Bonding Organocatalysts.

✍ Scribed by Daniel Pettersen; Raquel P. Herrera; Luca Bernardi; Franscesco Fini; Valentina Sgarzani; Rosario Fernandez; Jose M. Lassaletta; Alfredo Ricci


Publisher
John Wiley and Sons
Year
2006
Weight
21 KB
Volume
37
Category
Article
ISSN
0931-7597

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The use of 1 -fluoro-2,4-dinitrobenzene (FDNB) as a probe for the presence of hydrodisulfide groups in proteins is described. The technique involves derivatimtion of the protein with FDNB and subsequent treatment with dithiothreitol (DTT). The 2,4dinitrothiophenol (DNTP) released from the PDNB-deri