Use of 1-fluoro-2,4-dinitrobenzene as a
β
Tadashi Sawahata; Robert A. Neal
π
Article
π
1982
π
Elsevier Science
π
English
β 404 KB
The use of 1 -fluoro-2,4-dinitrobenzene (FDNB) as a probe for the presence of hydrodisulfide groups in proteins is described. The technique involves derivatimtion of the protein with FDNB and subsequent treatment with dithiothreitol (DTT). The 2,4dinitrothiophenol (DNTP) released from the PDNB-deri