A biosensor as warning device for the detection of cyanide, chlorophenols, atrazine and carbamate pesticides

✍ Scribed by Jean-Luc Besombes; Serge Cosnier; Pierre Labbé; Gilbert Reverdy

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 828 KB
• Volume: 311
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00686-g

✦ Synopsis

The determination of cyanide, chlorophenols, atrazine, dithiocarbamate and carbamate pesticides is described, utilizing an amperometric biosensor constructed by the electropolymerization of a pyrrole amphiphilic monomer-tyrosinase coating. Measurements were carried out with catechol, dopamiue, L-DOPA or epinephrlne as an enzyme substrate; the enzymatically generated quinoid products being electroreduced at -200 mV vs. SCE. The detection of these water pollutants was performed via their inhibiting action on the tyrosinase electrode. The characterization of the inhibition processes (competitive/non-competitive)

and their reversibility were examined. The detection limits are 0.4, 2, 2, 4 and 0.02 ,uM for 3,4-dichlorophenol, chloroisopropylphenylcarbamate, 3-chloroaniline, atrazine and cyanide, respectively.