A biological assay for intracellular SPP1 DNA

✍ Scribed by Klotz, G. ;Spatz, H. Ch.

• Book ID: 104773595
• Publisher: Springer
• Year: 1971
• Tongue: English
• Weight: 405 KB
• Volume: 110
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00438279

✦ Synopsis

Transfection with marker rescue techniques provides a sensitive assay for the DNA of phage SPP1. The method was used in a study of the process of replication of this phage. The data show that early during the eclipse, SPP 1 DNA increases exponentially, suggesting a geometric mode of replication. DNA replication ceases at the time of the appearance of the first intracellular phage particles.