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A BASIC program for the estimation of Michaelis-Menten parameters by the direct linear plot

✍ Scribed by John F. Brady; Hiroyuki Ishizaki


Book ID
113291125
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
143 KB
Volume
28
Category
Article
ISSN
0169-2607

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The performance of four parameter estimating procedures for the estimation of the adjustable parameters in the Michaelis-Menten model, the maximum initial rate Vmax, and the Michaelis-Menten constant Kin, including Lineweaver & Burk transformation (L-B), Eadie & Hofstee transformation (E-H), Eisenth

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The efficiencies of estimates obtained from the direct linear plot (A. Cornish-Bowden and R. Eisenthal, 1978, Biochem. Biophys. Acta, 523, 268) are shown to be dependent on the spacing of substrate concentrations. When substrate values are harmonically spaced, the direct linear plot should not be us