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A bank of frozen peripheral blood lymphocytes for In vitro immunological studies on east African cancer patients

✍ Scribed by C. M. Steel; A. G. Levin; T. Tsu; R. L. Gross

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
344 KB
Volume
13
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A bank of peripheral blood lymphocytes, frozen in liquid nitrogen, has been established from East African cancer patients and from control donors including healthy individuals and patients with non‐malignant disorders. The techniques of collection, lymphocyte separation and cell freezing are simple enough to have been applied successfully in an isolated mission hospital. In the first year of operation samples have been stored from over 400 individuals. On recovery, the cells have high viability, will respond to PHA in culture, are suitable for determination of HL‐A phenotype and react with sheep red blood cells in the spontaneous rosette test. It is predicted that lymphocyte banks of this type will have a useful place in the study of immunological factors in cancer, particularly in developing countries.

📜 SIMILAR VOLUMES

In vitro immunological studies on east a
In vitro immunological studies on east african cancer patients. II. Increased sensitivity of blood lymphocytes from untreated burkitt lymphoma patients to inhibition of spontaneous rosette formation
✍ R. L. Gross; A. G. Levin; C. M. Steel; S. Singh; G. Brubaker; F. G. Peers 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 483 KB

## Abstract Frozen aliquots of peripheral blood mononuclear cells from patients with Burkitt's lymphoma (BL) at various stages of the disease have been compared with samples from East African children who were healthy or who suffered from non‐malignant disorders. Using the sheep‐cell rosette test (

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## Abstract Spontaneous rosette formation was observed in eight out of nine Burkitt lymphoma biopsies. These were examined fresh and/or after culture in vitro for up to 48 h. The percentage of rosettes varied from 3.7% to 38%. There was a tendency for the percentage of rosettes to increase with tim