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A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system

✍ Scribed by Jill M. Weimer; Amelia Stanco; Jr-Gang Cheng; Ana C. Vargo; Santhi Voora; E. S. Anton

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
632 KB
Volume
56
Category
Article
ISSN
0894-1491

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✦ Synopsis

Abstract

Extracellular matrix associated Sparc‐like 1 (SC1/SPARCL1) can influence the function of astroglial cells in the developing and mature central nervous system (CNS). To examine SC1's significance in the CNS, we generated a BAC transgenic mouse model in which Sc1 is expressed in radial glia and their astrocyte derivatives using the astroglial‐specific Blbp (Brain‐lipid binding protein; [Feng et al., (1994) Neuron 12:895–908]) regulatory elements. Characterization of these Blbf‐Sc1 transgenic mice show elevated Sc1 transcript and protein in an astroglial selective pattern throughout the CNS. This model provides a novel in vivo system for evaluating the role of SC1 in brain development and function, in general, and for understanding SC1's significance in the fate and function of astroglial cells, in particular. © 2008 Wiley‐Liss, Inc.

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