Bone marrow-derived cells in the central nervous system of a mouse model of amyotrophic lateral sclerosis are associated with blood vessels and express CX3CR1

✍ Scribed by Coral-Ann B. Lewis; Jennifer N. Solomon; Fabio M. Rossi; Charles Krieger

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 449 KB
• Volume: 57
• Category: Article
• ISSN: 0894-1491
• DOI: 10.1002/glia.20859

✦ Synopsis

Amyotrophic lateral sclerosis (ALS) is associated with increased numbers of microglia within the CNS. However, it is unclear to what extent bone marrow (BM)-derived cells contribute to this microgliosis. We have studied the adoptive transfer of green fluorescent protein (GFP)-labeled whole BM cells and BM from mice that express GFP only in CX 3 CR11 cells (CX 3 CR1 1/GFP ) into the CNS of a murine model of ALS having over-expression of mutant superoxide dismutase (mSOD), and wt littermates. We find that most GFP1 and CX 3 CR1 1/GFP cells are found adjacent to the microvasculature within the CNS, both in mSOD and wt mice. GFP1 and CX 3 CR1 1/GFP cells within the CNS have a variety of morphologies, including cells with an elongated appearance, weak Iba-1 immunoreactivity, and often mannose receptor immunoreactivity, indicating that these cells are perivascular microglia. Typically, less than 10% of BMderived cells had a stellate-shape and expressed strong Iba-1 immunoreactivity, as expected for parenchymal microglia, indicating that BM-derived cells uncommonly generate parenchymal microglia. Adoptive transfer of BM-derived cells from CX 3 CR1 1/GFP mice revealed that many elongated cells are GFP1, demonstrating that some perivascular cells are derived from BM cells of the CX 3 CR11 lineage. The significantly greater numbers of BM cells in mSOD than in control mice indicate that the presence of these BM cells in the spinal cord is regulated by conditioning stimuli that may include irradiation and inflammatory factors within the CNS. V